Masterflex Single-Use Systems and Assemblies

Get your single-use bioprocess workflows up and running quickly. Let us customize your assemblies or select from our wide-range of products to build your own. Choose Masterflex Single-Use Custom Disposable Sterile Assemblies and eliminate assembling, sterilizing, and validating with just one call to us. Or, select every component to build your own assemblies. Either way, we offer the widest selection of trusted brands in the bioprocess market for tubing, fittings, gaskets, sanitary fittings and clamps, and more. Too much to choose from? Consult with our technical experts to help you get exactly what you need, so you can start reaping the many benefits of single-use systems: reduced cross-contamination risks, lower capital investment, no more cleaning and sterilizing, reduced turnaround times, scale an operation up or down with limited additional investment, and so much more.


Bioprocessing workflows rely on Masterflex

Masterflex offers the widest selection of trusted brands in the bioprocess market for tubing, fittings, gaskets, sanitary fittings and clamps, and more. Too much to choose from? Consult with our technical experts to help you get exactly what you need. Whatever your process needs—filtration, fluid lines, process containment, process control, cell generation, or sampling—you can count on Masterflex to supply the exact component you need to improve your efficiency.

Media Preparation: A vital step in the upstream process

media preparation workflow diagram image

Media prep is a vital step in the upstream process where the solution for cell culture is prepared. It requires significant time and effort with innovative approaches including the use of single-use technology throughout the entire process. Each approach is individually configured based on the manufacturers’ application. Peristaltic pumps play an important role for dosing the exact amounts of process nutrients and other media using high purity, flexible tubing. Peristaltic pumps can also gently recirculate the cells in the solution and protect cell viability.

Fermentation: Growing microorganisms for the biochemical synthesis of organic compounds

Fermentation workflow diagram image

Industrial fermentation involves a sample a tank of controlled pH, aeration, agitation, temperature. It requires more preliminary steps than other bioprocess workflows. Common microorganisms used in this process are Chinese hamster ovary cells (CHO), yeast, fungi, and bacteria. Masterflex® peristaltic pumps are useful in this workflow and are recommended over diaphragm pumps. Peristaltic pumps provide accurate dosing capabilities and, unlike diaphragm pumps, have an isolated and unconvoluted fluid path. This makes maintenance easy and ensures no product gets stuck in a crevice.

Harvesting: Collection or clarification of product from fermentation

harvesting workflow diagram image

Typical harvest methods include Tangential Flow Filtration (TFF) or centrifugation as a means of concentrating the target compound. In either of these methods, quality and purity are paramount. Wetted materials (any material touching the fluid) must be validated USP Class VI and sterilized. Peristaltic pumps remain accurate at higher pressures, making them ideal for filtration centric workflows.

Purification: Removal of impurities from the harvest and clarification workflows

purification workflow diagram image

Affinity and ion-exchange chromatography are used to remove metals and reaction by-products along with additional methods of filtration. Recently, single-use tube component assemblies have become more prevalent in this workflow as higher throughputs and versatility on the process floor are demanded. The isolated fluid flow path of peristaltic pumps, such as Masterflex® pumps, is critical to this workflow. These pumps mitigate the potential for contamination and reduce the required material validation.

Fill-Finish: Gently transfer sterile product for packaging or lyophilization

fill-finish workflow diagram image

This critical process — highly regulated with global regulatory and compliance requirements — transfers the sterile product, now in its most valuable form, to a sterile vial or bottle before it is packaged and distributed or sent to lyophilization (freeze-drying) before packaging. Peristaltic pumps are the top choice for their ability to provide easy change-overs, reduce cross-contamination, and gently pump shear-sensitive fluids, large molecules, and live cells. The valuable end product is contained in the tubing, never touching any part of the pump. Our single-use sterilized assemblies, developed specifically for this process, eliminate downtime for cleaning.

Its time to make your process more efficient

  • 1Exactly what you need
    Choose from thousands of components. We custom configure, validate, and package according to your specifications.
  • 2Exactly when you need it
    Stop cleaning and revalidating your system. We manage your inventory and send assemblies according to your schedule.
  • 3Call for a Quote today
    Masterflex Single-Use experts provide a quote with full schematic, followed by an actual sample for your approval

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